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For effective separation of the N-TFA n-butyl ester amino acids on the stainless steel column by GLC, dual column of the mixed stationary phases. 3.36% OV-17 + 3.0% SE-30 (column 1) and 1% NPGS+0.5% OV-17+0.5% 5E-30(column 2)on chromosorb W HP 100-120 mesh, were used. On the column 1, the nineteen amino acids except histidine were obtained. However, alanine and valine peaks were not separated by this column. On the column 2, the sixteen amino acid peaks showed good separation, but tryptophan. arginine, histidine, and tyrosine peaks were not obtained. Calibration graphs for all amino acids obtained by the plotting the ratios of their peaks hights to that of internal standard versus the micro mole of the amino acids in the range 1.25 x 10^(-3) μ mol-1.0 x 10^(-2) μmole showed linearity and passed through the origin.
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